Category Archives: Ask a guru answer

Issue with Column Arrangement in Data Review-How to Avoid and How to Fix

Some have experineced an issue when starting the application after rearranging columns in data review.

They recieve a .Net framework error that states that columns have been arranged to the left of frozen columns.

This is a known issue that will be fixed in the next version of TraceFinder.

To avoid the issue the columns in the image below should remain the farthest to the left on the data review grids.

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If you experience the issue, rename the file tracefindersettings.sqlite to TraceFindersettings_old.sqlite and restart the application.

The factory default settings  backup file will be automatically recreate the tracefindersettings.sqlite file.

The image below shows the directory path to the file.

Note that the directory path is dependant on the version of TraceFinder that it is installed.

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Isotope Pattern Scoring in TF 3.1 – Answer to “Ask A Guru” Question

Below is an excerpt from the online help in TF 3.1.

A few questions have come up on how we calculate the isotope pattern score and visualization.

Here is the answer directly from the online documentation. Remember we do have comprehensive manuals and help under the Help Menu.

Link to PDF file: isotope scoring

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Reminder of How to Import a Custom Reporting Template

We have had a alot of custom report request coming in over the last few weeks, and a few people have asked how to import them for use.

Below is a link to a Post last year on how to bring in a template and use it in TF.

https://mytracefinder.com/2012/11/08/importing-a-new-custom-report-template/https://mytracefinder.com/2012/11/08/importing-a-new-custom-report-template/

Applying an external/historical curve to a batch of samples after Acquisition – Ask A Guru

The video below shows how to apply a calibration curve from one batch that contains calibration samples to a batch of samples that contain no calibrators.

This workflow is the same as selecting the external curve in the Acquisition workflow, but may be used on any batch of samples after acquisition.

The features allows the user to run a calibration batch in the morning and use that curve for all subsequent batches for the time period their SOP allows.

HMMMMMM… Maybe your day just got alittle easier.

If the video is blurry please click the cog wheel at the bottom of the panel and increase the video display resolution.

Storing and Using Data on a Mapped Network Drive

Recently, an “Ask A Guru”, question came through asking how to have data on any other drive besides C:.

 

In the video below, the process of how to set the root directory structure that TraceFinder needs to find data on any drive is shown.

Once created you can move whole projects with the Windows Explorer tool, or you can  use either the “Save As” to make a copy or the “Move” File-Menu item top move the batch to the new disk.

If you have a network shared directory, that you want to use, simply map that drive to your windows directory. It will then function as any other attached drive with the exception of being able to acquire data across the network.

If the video is blurry please click the cog wheel at the bottom of the panel and increase the video display resolution.

 

TF 3.0 – The Daily Technician Workflow – Point, Shoot and Read the report

Part of what makes TraceFinder a tool for the average chemist, is that complex experiments and processing can be setup once.

But then it can be used a thousand times, by technicians that just need to make samples and put them on the instruments to run.

Though there are many other things that can be done. Although, this isn’t an open access software, we have made it easy to just run your samples and get a report out on the fly that can go to a printer or to a pdf after each injection.

Please view the video below.

If the video is blurry please click the cog wheel at the bottom of the panel and increase the video display resolution.

TF 3.0 – How to add Adducts for use in the Compound Database

The video below shows how to edit the positive and negative adducts that can be used in the CDB.

The use of adducts becomes very useful in calcualtions of the exact masses for screening and high resolution quantitation.

This also helps in the screening qualitiy measurement of isotope confirmation and identification.

If the video is blurry please click the cog wheel at the bottom of the panel and increase the video display resolution.

How to do Background Subtraction w/ Qualbrowser

A couple of questions have come in, on how to best perform background or blank subtraction.

QualBrowser has a native tool to perform this task.

Please see the video below, on how to perform the operation on a single sample or a batch of samples using a .sld file.

If the video is blurry please click the cog wheel at the bottom of the panel and increase the video display resolution.

The Master Method – A trinity of information

We will start a short series on the Master Method. So here we go.

Think of the Master Method as the supply truck that comes everytime you want to run an assay. It contains what you need to get things done. It’s alittle bulky and can be somewhat intimidating the first time you see it. Since it controls alot of the available power TF gives you, it has lots that can be,but doenst have to be configured and used.

For example, if a value is set to zero in the QAQC section it isnt turned on for use by the flagging system.

But lets start off with the basics.

The Master Method is a repository for the three things needed to gather and process a data file.

  1. The instrument method
  2. The peak processing parameters
  3. The selected reports to be produced.

With these three things we can submit a batch of samples and acquire, process and produce reports on the fly, after every sample. (but you need a sample list too)

The instrument method is a copy of a stored instrument method somewhere on your hard disk. It is associated with the processing method and can be changed according your your needs. But you always have the ability to go back to your orginal instrument method and copy over any changes. It’s a safety net.

It also insures that you only have to pick the Master Method when making a batch of samples. This keeps a batch consistant, run after run and technician to technician.

The processing parameters are set up for each peak and compound grouping. Once set up again it keeps things consistant.

Thirdly, is the report section. This seems to be a bit of confusion at times. By selecting which reports and what format you want them produced in, ahead of time. The data is always produced in the same way without missing a page. It’s also nessary for the reporting on the fly feature.

If you want to create reports after each injection, simply check the box on batch submission that says “Create Reports”. Then however you selected the report to be produced and one or multiple formats, it make them once the sample has been acquired and automatically processed. Except for Batch level reports, which are produced at the end of the batch sequence.

Follow the video below to see the process.

The video is from the soon to be release TF 3.0 but the workflow is the same.

If the video is blurry please click the cog wheel at the bottom of the panel and increase the video display resolution.

Extending a Calibration Curve “Creating a historical cal file” – (Answer to an “Ask a Guru” question

One aspect of the applied market labs is that a calibration curve does not have to run with each batch of samples.

Previously we showed you how to associate the calibration file from one batch to the one you are currently working with.

In this video, we show you how to create a historical curve by extending a calibration curve from another batch.

By selecting the curve and the extend calibration function, the points found in the current batch of samples will be added to the points from the previously acquired batch of samples. This in conjuntion with turning on and off groups of compounds can allow for the user to build calibration curves for large sets of samples and use a historical running average of calibrators.

The video is from the soon to be release TF 3.0 but the workflow is the same.

If the video is blurry please click the cog wheel at the bottom of the panel and increase the video display resolution.