The Master Method – A trinity of information
We will start a short series on the Master Method. So here we go.
Think of the Master Method as the supply truck that comes everytime you want to run an assay. It contains what you need to get things done. It’s alittle bulky and can be somewhat intimidating the first time you see it. Since it controls alot of the available power TF gives you, it has lots that can be,but doenst have to be configured and used.
For example, if a value is set to zero in the QAQC section it isnt turned on for use by the flagging system.
But lets start off with the basics.
The Master Method is a repository for the three things needed to gather and process a data file.
- The instrument method
- The peak processing parameters
- The selected reports to be produced.
With these three things we can submit a batch of samples and acquire, process and produce reports on the fly, after every sample. (but you need a sample list too)
The instrument method is a copy of a stored instrument method somewhere on your hard disk. It is associated with the processing method and can be changed according your your needs. But you always have the ability to go back to your orginal instrument method and copy over any changes. It’s a safety net.
It also insures that you only have to pick the Master Method when making a batch of samples. This keeps a batch consistant, run after run and technician to technician.
The processing parameters are set up for each peak and compound grouping. Once set up again it keeps things consistant.
Thirdly, is the report section. This seems to be a bit of confusion at times. By selecting which reports and what format you want them produced in, ahead of time. The data is always produced in the same way without missing a page. It’s also nessary for the reporting on the fly feature.
If you want to create reports after each injection, simply check the box on batch submission that says “Create Reports”. Then however you selected the report to be produced and one or multiple formats, it make them once the sample has been acquired and automatically processed. Except for Batch level reports, which are produced at the end of the batch sequence.
Follow the video below to see the process.
The video is from the soon to be release TF 3.0 but the workflow is the same.
If the video is blurry please click the cog wheel at the bottom of the panel and increase the video display resolution.