Internal Standard Retention Time and Peak Width Diagnostic Chart
This a useful and simple report that is best viewed in Excel.
Click here to download the zip file:
Place in the folder: C:\TraceFinderData\4.0\Templates\ReportTemplates
The chart shows the Retention Time and the Chromatographic Peak Width at Half Height, in a line chart over the entire batch. It should show you the performance of the internal standard and the reliability of the chromatographic conditions. A moving retention time and/or a widening of the chromatographic peak may indicate issues with the columns or mobile phase. This is useful in determining the health of the system and variability of the assay over time.
The Chromatographic Peak Width can be attained by setting the conditions in the detection portion of the method as shown below. You can change the width measurement to be any desired value. Default is 50% of peak height, though 25% may better gauge peak fronting or tailing.
The report is currently set up to show 59 samples from the data table. If your data set is typically large or smaller you can adjust the ending row in the series section of the chart dialog. Simply open the editor for this report, double click on the chart and the dialog will open as shown below. Expand the section for the Data Source/Series and enter your typical ending row. Any empty cells will not be charted.