Multiplexing methods: Bigger is definitely not better.
So if you’re getting an error that in the instrument log that says. Something like, “data file closed before the instrument was finished.”
And you’re using an Aria multiplexing front end.
Then most likely the mass spec portion of your method file has an acquire time larger than the data window in the Aria portion of your method.
This could cause the raw files to remain open and use up RAM depending on your batch size you could run out of memory depending on what version you are using.
RULE OF THUMB: The mass spec acquisition time must be EQUAL TO OR LESS THAN, the Duration time in the Aria method. So if the duration time is 3.0 minutes then make your mass spec time 2.99.
So, you can definitely do more with less.
Posted on May 25, 2012, in Acquisition, Method Development, Troubleshooting. Bookmark the permalink. Leave a comment.
Leave a comment