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TraceFinder 3.2 – Method Setup Tweak – Threshold Setting

There have been a few questions on the issue of peaks not being integrated, while performing quantitation, although the signal is strong. A reason could be that when a compound is created in the method, it has a related compound database entry for a threshold setting. This setting is used in targeted screening but is not editable in quantitation mode.

The setting has a default value of 5000 for calculated peak area. This setting does have a global override in the Local and Master method  Processing section. The default value is unchecked and blank. This means the default threshold is 5000 for all peaks. If you are having an issue where the peak is not integrated but has a strong signal, then change the setting by checking the box and entering a new value such as 0 or 500. The data will need to be reprocessed as this value is not automatically applied.

Threshold 1

threshold2