A question came across the Blog that asked about Licensing and Multiplexing?
The answer to the question is two.
One License for the version of the application(ie. TraceFinder EFS, TraceFinder Clinical, ect..) and One for the Additional Module such as Multiplexing support for the Aria OS.
IF you go to the upper left section of the application in the menu bar and select Help, at the bottom of the dropdown will be an item called License Activation.
Here you can open the licensing window and request or enter license data.
If your trial period is over then only a license screen will appear when you open the application from the file menu or from the desktop icon.
This is true for both the Application and the Additional Feature of Multiplexing.