Even though its the most memorable line from the movie “Highlander”, this statement has come up many times for assays where semi quantitation occurs in screening analysis.
One way to accomplish a rough estimate calculated amount is to use the ability to use a single calibration level for a compound.
When you do this there are two settings in the Method setting that can be used.
Selecting Average RF allows for the average response factor for a calibration curve to be calcualted. If you use cal one point
the response of the point will be compared to the response of the compound in the sample and a rough estimate based on the
average will estimate the calcualted amount.
If using a linear curve and one point, you MUST set the Origin setting to Force. This sets the origin at Zero and draw the curve
the response of the one calibration point. Thereby giving you a curve to estimate the calcualted amount from.
There is a third way using Internal Standards, but we’ll save that for another day.