Disappearing Peaks – Master Method and Instrument Method not set correctly together
Last week at a customer site, the system was collecting information but no peaks were being detected and displayed.
If you opened the Raw files up in Qual browser and applied a layout AND selected autofilter the peaks for the compounds would appear correctly.
The issue was that the instrument method that had been updated in the master method did not collect information for the set quan mass. that is the filters in the rawfile looked something like this MS + SRM 906.01 [59.2-60.2] but the filter selection stored in the master method looked like this MS + SRM 906.01 [59.3-60.3], and the extracted mass set in grid was set to 59.27.
Video of update filter