FreeStyle tutorial: Automated Peak Detection settings
The video below demonstrates how to setup automated peak detection settings inside of a workspace, through annotated click-through text.
Freestyle tutorial: Comparing Chromatograms
The video below demonstrates how to compare chromatograms in a workplace, through annotated click-through text.
FreeStyle tutorial: Background Subtraction
The video below demonstrates how to perform background subtraction, through annotated click-through text.
FreeStyle tutorial: Elemental Composition
The video below demonstrates how to determine the elemental composition of a spectral peak, through annotated click-through text.
FreeStyle tutorial: Displaying Mass Spectra
The video below demonstrates how to mass spectra inside of a workspace, through annotated click-through text.
FreeStyle tutorial: Averaging Spectra
The video below demonstrates how to average mass spectra inside of a workspace, through annotated click-through text.
Video – How to create NIST 2014 custom libraries with FreeStyle
To make custom libraries for use in TraceFinder 3.3 Unknown Analysis, the easiest way is to curate your spectra with FreeStyle and send to the NIST 2014 application which comes with the purchase of any NIST 2014 library.
Use FreeStyle to Create NIST 2014 Spectral Library Entries
TraceFinder 3.3 utilizes both the Thermo Library Manager and NIST 2014 HRAM library search system for confirmation and identification of found chromatographic peaks. To support the creation of HRAM libraries with NIST 2014 there are a few things to setup.
First, acquire NIST 2014 from a NIST distributor. Thermo has the rights to distribute via purchase this application. Also, FreeStyle 1.1SP1 will be needed.
Once installed there you must set up the spectra import default settings to match the decimal place criteria you plan on using for the type of data contained in your RAW file.
Next you must have Thermo FreeStyle 1.1SP1 installed.
Then you must set up two settings for data export to NIST.
Under the File menu, select File there is an OPTIONS button to click on. Then select Mass Precision and set the number of decimal places for the data type you will be using.
Next select the Library Search option and set the default to be MSMS or In-Source.
Save the settings. (Note: in some instances FreeStyle must be restarted for the settings to be implemented)
The next step in the process is to open the data file(s) you want to use to create library entries. Select the Workspace Processing selector and click the Library Manager button. Select the Library you want perform an initial search against. This must be done even if you are creating your own library.
This is followed by working up your data to reflect the spectra you wish to utilize and then clicking the Export to NIST button, while the spectra panel is highlighted. The NIST search panel will open and display an initial search result.
Next select the Librarian tab on the bottom of the screen and select edit button in the middle toolbar to open the editing panel; make adjustments such as changing the name of the spectra to be entered. Then select the Add to Library button finalize with the library file selection.
Thermo Freestyle – General Navigation Tutorial
In the pending TraceFinder 3.2 release, FreeStyle will be an optional feature that comes with every installation.
FreeStyle is a new qualitative viewing package that is taking on the features of QualBrowser.
TraceFinder 3.2 gives you the ability to utilize both QualBrowser and Freestyle when analyzing data, from links within the application.
Please view the video below as a quick tutorial on the basic navigation of FreeStyle.
Video player has changed. If the picture appears blurry, in the top right hand corner of the player you can enable HD to play back high resolution video.
Note: FreeStyle is a separate application and will require a separate license from TraceFinder.
However, when you apply for a TraceFinder 3.2 license you will receive both a TF license and a FreeStyle license.
You simply apply each to their respective license when using the applications together.